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. 2010 Apr 26;120(5):1617–1626. doi: 10.1172/JCI41678

Figure 6. Application of 9-HODE and heat (43°C) demonstrate similar latencies for activation of TRPV1.

Figure 6

(A) The latencies for generation of single-channel inward currents due to application of either 9-HODE (100 μM) or 43°C to TRPV1-expressing CHO cells. n = 6–10. Recording was carried out in cell-attached (C-A) and inside-out (I-O) configurations (configur.). (B) Representative single-channel inside-out traces illustrating the measurement of the latency for 9-HODE. Latency was calculated as the difference between a and b. A solution containing potassium (50 mM) was applied via the recording pipette to measure the inherent latency of the perfusion system (b). Inside-out configuration was established for 3–5 minutes. c is a close state. 1, 2, and 3 are numbers of channels in the patches. (C) Representative single-channel inside-out traces illustrating the measurement of the latency for 43°C. Temperature was applied immediately (within 1 minute) after establishment of the inside-out configuration. Latency was calculated as a–b, where b is the time to reach 43°C in the vicinity of the cells. Temperature ramp was recorded by placing a thermistor in the position of previously recorded cells.