Figure 7. Indirect activation of TRPV1 by linoleic acid.
(A) Concentration-dependent effect of linoleic acid (LA) on Po of TRPV1 in transfected CHO cells using inside-out configuration at room temperature. Patches were washed at least 3 minutes before linoleic acid application at room temperature. (B) Representative single-channel traces demonstrating the effect of different concentrations of linoleic acid on Po of TRPV1-expressing CHO cell membrane patches. c is a close state. o is an open state, in which the patch contained 1 channel. 1, 2, and 3 are the numbers of channels in the patches. (C) Effect of pretreatment with either vehicle or NDGA (30 μM, 15 minutes) on linoleic acid–induced activation of TRPV1 transfected CHO cells as measured using calcium imaging (n = 25–50; P = 0.0001). **P < 0.01; ***P < 0.001.