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. Author manuscript; available in PMC: 2010 Jul 7.
Published in final edited form as: Cancer Cell. 2009 Jul 7;16(1):9–20. doi: 10.1016/j.ccr.2009.04.009

Figure 3. Serum-induced chemotaxis in glioma and prostate cancer cells is dependent on Akt-mediated phosphorylation of EphA2 at S897.

Figure 3

A–B. Overexpression of WT-EphA2 and S897A-EphA2 in the indicated cell lines following retroviral infection.

C–D. Chemotaxis towards serum was promoted by overexpression of WT-EphA2 but not S897A-EphA2 in PTEN-null tumor cells. Numbers represent mean ± S.D. from 6 random fields. *, p<0.05; **, p<0.01; ***, p<0.001.

E–H. Restoration of PTEN expression in PTEN–null U373 cells inhibited basal cell migration. (E) PTEN re-expression significantly reduced FBS-induced Akt activities, which was quantified in F. In both Boyden chamber migration (G) and scratch wound (H) assays, PTEN restoration reduced cell migration. Numbers represent mean ± S.D. from 6 random fields. Scale bar, 100 μm.