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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: J Clin Gastroenterol. 2007 Jul;41(Suppl 2):S226–S242. doi: 10.1097/MCG.0b013e31803233b7

FIGURE 3.

FIGURE 3

Illustrations of purified parietal cell compared with whole gastric epithelium microarray data and immunostaining of K and Cl channels in rabbit gastric glands. On the top left is the image of the microarray showing in red fluorescence the spot corresponding to KCNQ1 and on the bottom left the spot corresponding to KCNE2 where the red fluorescence indicates higher expression in the parietal cell transcriptome. In the middle, there is the confocal image of staining with anti-ATPase α subunit in red and against KCNQ1 in green, showing their localization in both resting and stimulated glands. On the right top right is the microarray image showing high expression of CLIC6 in the parietal cell and then immunostaining of CLIC6 and ATPase bottom images and a fused image top right. There appears to be some colocalization of the channels with the ATPase in the parietal cell but particularly intense staining for CLIC6 in the lumen of the gastric gland.