Figure 3. Phenotypic analysis of eat-6 RNAi and overexpression.

(a) Relative reciprocal T₅₀ values of aldicarb-induced paralysis of animals untreated (black bars) or pretreated with 5-HT (hatched bars). The error bars indicate SEM (n>3 replicates). Both RNAi and overexpression of eat-6 conferred hypersensitivity to aldicarb compared to WT (* p<0.05, ** p<0.01). Pretreatment of 5-HT reduced aldicarb sensitivity in WT animals (p<0.005), but not in eat-6 RNAi animals. Student's t-test. (b) The ratio of the amplitude of the excitation and recovery peaks (black bars, left axis), and the fraction of EPSPs that failed to trigger APs (hatched bars, right axis). The error bars indicate SEM (n = 15). (c–h) Representative EPG recordings. EPSP designates an excitatory postsynaptic potential, E designates the peak corresponding to the muscle excitation (depolarization), R designates the peak corresponding to muscle recovery.