Figure 3. Growth and differentiation potentials of ESP and EMP cells.

(A) Fluorescence images of DiI-labeled ESP cells co-cultured with DiO-labeled EMP cells following Hoechst DNA dye staining. Bars, 200 µm. (B) Cloning efficiencies of ESP cells and EMP cells in EGM-2MV medium. * P<0.001. Each bar indicates the mean ± SEM. N ≥4. (C) Phase contrast micrographs and fluorescence images (insets) of colonies generated from ESP and EMP cultures. The ESP cells and EMP cells were separately seeded at a clonal density, cultured in EGM-2MV medium for two weeks, and subjected to immunofluorescence studies using antibodies against the indicated markers. CK, cytokeratin. Bars, 500 µm.