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. 2010 Apr 1;11:54. doi: 10.1186/1471-2350-11-54

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Copyright ©2010 Paradis et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Polynomial graphic representation of the standard curve (A) and quantitative detection of JAK2V617F from specimens (B). Typical representation of the delta MFI curves and bar graphs applied to the detection of JAK2V617F variant from specimens. The percentage of mutated alleles is determined by comparing the height of the bar graphs from specimens with the standard curve. The equation is Y = 0.0473X²-12.6X+256.