Table 1.

Primers and probes used in end point PCR, ASPE and DH reactions.

PCR	Forward primer	5'-AGCAAGCTTTCTCACAAGCA-3'
	Reverse primer	5'biotin-CTGACACCTAGCTGTGATCCTG-3'
DH	Wild-type probe	5'unilinker-TGGAGTATGTGTCTGTGGAGA-3'
	Mutant probe	5'unilinker-TGGAGTATGTTTCTGTGGAGA-3'
ASPE	Forward primers	5'-AGCATTTGGTTTTAAATTATGGAGTATATT-3'
		5'-AGCATTTGGTTTTAAATTATGGAGTATGTG-3'PO4
	Reverse primer	5'biotin-CTGACACCTAGCTGTGATCCTG-3'

The reverse primer is biotinylated at its 5' end. The probes differing by a single nucleotide (bold letter) contain a unilinker at their 5' ends for coupling to the microspheres.