Fig. 5.

Neither the full-length anti-IgE aptamer nor a construct modified via the addition of a 10-base polythymine linker to increase flexibility supports efficient E-AB signaling. In contrast, a destabilized sequence (through point mutation) supports at least modest E-AB gain. The destabilized aptamer produces a signal change of 20% at saturating IgE concentrations (200 nM) with an apparent affinity of 18 ± 3 nM.