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. 2010 Mar 17;30(11):3973–3982. doi: 10.1523/JNEUROSCI.6421-09.2010

Figure 2.

Figure 2.

A, p53 deficiency does not inhibit camptothecin-induced upregulation of Sertad1. Cortical neurons from p53+/+ and p53−/− mice were treated with and without camptothecin for 8 h. Left, Sertad1 levels were then analyzed by RT-PCR. S12 was shown as a control for equal input. Right, Densitometric analysis of Sertad1 signals normalized against S12 signals. B, E2F-1 downregulation partially blocks camptothecin-induced upregulation of Sertad1. Cortical neurons were transfected with E2F-1 and control siRNA and were treated with and without camptothecin for 8 h. Left, Sertad1 levels were then analyzed by RT-PCR. S12 was shown as a control for equal input. Right, Densitometric analysis of Sertad1 signals normalized against S12 signals. Significance comparisons with untreated controls: *p < 0.05. Error bars indicate SEM. C, Western blot analyses showing downregulation of E2F-1 in cells treated with E2F-1 siRNA compared with control siRNA. Actin is provided as loading control.