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. Author manuscript; available in PMC: 2011 Apr 19.
Published in final edited form as: Vaccine. 2010 Mar 1;28(18):3219–3230. doi: 10.1016/j.vaccine.2010.02.022

Fig. 6.

Fig. 6

IgA (A) and IgG (B) Ab-forming cell (AFC) responses by mice nasally vaccinated with LTN/F1-V DNA vaccine. Mice were nasally dosed and boosted with DNA and F1-Ag protein vaccines, as described in Fig. 4, and lymphoid tissues were isolated on wk 14. Total splenic, head and neck lymph node (HNLN), NALT, nasal passage (NP), submaxillary gland (SMG), small intestinal lamina propria (iLP), and Peyer's patch (PP) mononuclear cells were isolated from each DNA vaccine group (5 mice/group/experiment) and evaluated in a B cell ELISPOT assay to assess F1-Ag- and V-Ag-specific (A) IgA and (B) IgG AFCs, as well as total (A) IgA and (B) IgG AFCs. Depicted is the mean ± SEM of AFC responses taken from two experiments.