Skip to main content
. 2010 May;176(5):2177–2187. doi: 10.2353/ajpath.2010.090759

Figure 4.

Figure 4

The infarcted myocardium mobilizes CCR5+ mononuclear cells. A: Flow cytometry analysis of the infarct infiltrate revealed that a significant percentage of mononuclear cells infiltrating the infarcted heart after 24 hours of reperfusion are CCR5+ (38.3%+3.7 of the CD45+ cells isolated from the infarct are CCR5+). B: As expected, CCR5 KO infarcts had no CCR5+ cells. C: In wild-type mouse infarcts a large percentage of the CCR5+ cells (38.25 ± 3.3) were identified as CD14+ monocytes. D: A significant number (3.73% ± 0.37) of the CCR5+ cells were CD4+ T cells. E–F: CCR5 expression in CD4+ T cells was associated with expression of the Treg-specific marker foxp3. The CD4+/CCR5+ subset (F) had a higher percentage of foxp3+ Tregs than the CD4+/CCR5− population (E) (percentage of foxp3+ Tregs, CD4+/CCR5+ subpopulation: 45.49 ± 4.38 versus CD4+/CCR5− subpopulation: 12.47% ± 1.45, P < 0.01). The dot plots and percentages reflect a representative experiment pooling 2 infarcted hearts. Statistical analysis was based on three independent experiments using 6 infarcted hearts.