Figure 6.

Heterogeneous mesenchymal inductions by neonatal keratinocytes. A: Cytokine-induced plasticity of the neonatal epidermal keratinocytes was demonstrated by phase contrast microscopy, after treatments for 3 days. B: Under TNF-α, most of the epithelial cells are converted to vimentin (green) and FSP1 (red) double-positive cells (yellow). In response to TGF-β1, two populations of mesenchymal-positive cells are emerged, either as the double-positive ones, or vimentin-positive/FSP-1–negative ones. C: Mesenchymal induction was measured by Western blot for vimentin and FSP1. D: Motility of the primary keratinocytes under mesenchymal transition was measured by in vitro wound healing, and determined by closure of the scratched tracks (n = 4, *P < 0.05 as significance by Student t test).