Table 1.

Real-time RT-PCR primers

Gene	Sequence	Primer size	Tm (°C)
TNF-α	F: 5′-AAGCCTGTAGCCCATGTTGTA-3′	21 mer	52
	R: 5′-TCAGCTCCACGCCATTG-3′	17 mer	49
MMP-9	F: 5′-GGGAGACGCCCATTTCG-3′	17 mer	52
	R: 5′-CGCGCCATCTGCGTTT-3′	16 mer	49
MMP-13	F: 5′-CAGACTTCACGATGGCATTGC-3′	21 mer	54
	R: 5′-AGGAAAAGCATGAGCCAGCA-3′	20 mer	52
Collagen I	F: 5′-CAGCCGCTTCACCTACAGC-3′	19 mer	55
	R: 5′-TTTTGTATTCAATCACTGTCTTGCC-3′	25 mer	53
α-SMA	F: 5′-CGTGGGTGACGAAGCACAG-3′	19 mer	55
	R: 5′-GGTGGGATGCTCTTCAGGG-3′	19 mer	55
Twist-1	F: 5′-TGAGCAACAGCGAGGAAGAGC-3′	22 mer	52
	R: 5′-TGAATCTTGCTCAGCTTGTCCG-3′	22 mer	50
Slug	F: 5′-ACAAGCAGCTGCACTGTGAT-3′	20 mer	55
	R: 5′-ACACAAGGCAATGTGTGGGT-3′	20 mer	55
E-cadherin	F: 5′-ACAACAAGCCCGAATTCACCCA-3′	22 mer	55
	R: 5′-TCACAGCTGTTGCTGTTGTGCT-3′	22 mer	55
N-cadherin	F: 5′-TCATTGCCATCCTGCTCTGCAT-3	22 mer	55
	R: 5′-AGTTGTTTGGCCTGGCGTTCTT-3′	22 mer	55
Vimentin	F: 5′-AAAGTGTGGCTGCCAAGAACCT-3′	22 mer	55
	R: 5′-ATTTCACGCATCTGGCGTTCCA-3′	22 mer	55
BMP2	F: 5′-AACACTGTGCGCAGCTTCC-3′	19 mer	55
	R: 5′-CTCCGGGTTGTTTTCCCAC-3′	19 mer	55
GAPDH	F: 5′-GAAGGTGAAGGTCGGAGT-3′	18 mer	50
	R: 5′-GAGATGGTGATGGGATTTC-3′	20 mer	50