Figure 5.

Expression and secretion of Angptl2 in primary RA synovial fibroblasts. A: Comparison of the relative expression levels of Angptl family and angiopoietin-1 (Ang1) and -2 (Ang2) in RA synovial tissue (n = 8) estimated with a Quantitative RT-PCR analysis. Closed columns indicate relative mRNA levels normalized to β-actin mRNA levels. The data of Angptl2 was set to 100%. Columns represent the means ± SD. B: Concentrations of cultured synovial tissues–derived Angptl2 protein secreted into the medium were determined by ELISA at 0 hours (medium before starting culture), 6 hours, and 24 hours after starting the culture (n = 5).