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. 2010 May;176(5):2378–2384. doi: 10.2353/ajpath.2010.091036

Figure 2.

Figure 2

Tissue distribution of SUSD4 by Western blot. A: Membrane protein extracts of murine brain (lane 1), eye (lane 2), spinal cord (lane 3), testis (lane 4), thymus (lane 5), liver (lane 6), kidney (lane 7), stomach (lane 8), lung (lane 9), heart (lane 10), intestine (lane 11), and muscle (lane 12) and the purified SUSD4 protein (positive control, lane 13) were separated by 10% SDS-PAGE and probed with SUSD4 antiserum. An ∼80-kDa band was detected in brains and spinal cords, whereas an ∼50-kDa band was detected in eyes and testes (arrows). No band was detected in the other tissues. Control experiments using the same dilution of preimmunization serum showed no band (data not shown). B: After probing, the membrane was stained with Coomassie Blue to show relative amounts of loaded protein.