Table 1.
Time | Category | Noninjected | MO - Control | MO - SUSD4 |
---|---|---|---|---|
3 dpf | Mortality | 2.6% (4 of 150) | 6.0% (9 of 150) | 8.0% (12 of 150) |
Abnormality | 1.3% (2 of 146) | 3.5% (5 of 141) | 28.7% (43 of 138) | |
5 dpf | Mortality | 2.6% (4 of 150) | 6.0% (9 of 150) | 12.7% (19 of 150) |
Abnormality | 1.4% (2 of 146) | 4.0% (6 of 141) | 27.5% (36 of 131) | |
7 dpf | Mortality | 2.6% (4 of 150) | 6.7% (10 of 150) | 14.0% (21 of 150) |
Abnormality | 1.4% (2 of 146) | 5.7% (8 of 140) | 28.7% (37 of 129) |
Two nanograms of nonspecific morpholinos (MOs) or SUSD4-specific MOs were injected into 150 zebrafish embryos at one- or two-cell stage; surviving fish with morphological or locomotive abnormalities were counted at 3, 5, and 7 dpf (representative results of multiple experiments).