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. 2010 May;176(5):2405–2413. doi: 10.2353/ajpath.2010.090704

Figure 1.

Figure 1

Transgenic mice and GFP expression in vitro. A: Breeding approach to generate oim/oim;3.6GFP and oim/oim;2.3GFP mice. Heterozygous oim/+ mice were bred with mice homozygous for a GFP marker. The F1 progeny (heterozygous for both transgene [GFP] and for an OIM mutation) were selected to breed with homozygous GFP mice to obtain homozygous GFP mice with one allele harboring an OIM mutation. These mice were bred to obtain experimental mice either wild-type for the mutation (+/+) or homozygous for an OIM mutation (oim/oim) in a homozygous GFP background. B: GFP expression in marrow stromal cultures (MSCs) derived from 3-month-old wild-type or oim/oim mice. A series of images was obtained with a concatenated image covering 63% of the area of a 35-mm tissue culture well. The left panel shows 3.6GFP expression in 7-day-old cultures. No major differences were observed between wild-type cultures and oim/oim cultures. The right panel shows 2.3GFP expression in day 17 cultures. Large areas of GFP-expressing cells were detected in the +/+;2.3GFP cultures. In contrast, only a few GFP expressing colonies were observed in the oim/oim;2.3GFP cultures.