Figure 4.

Osteoclast formation and function in vitro from wild-type and oim mice. A: Representative images of cocultures of mouse calvaria osteoblasts (mCOB) with bone marrow–derived nonadherent cells (Bmmc) from +/+ or oim/oim mice. B: Quantifications of the Trap-positive multinucleated osteoclasts formed in these cultures. Significantly more osteoclasts were formed when the BMMC-derived osteoclast progenitors were cocultured with oim/oim mCOBs. C: Quantification of the area of osteoclast resorption pits in calcium precoated osteologic plates. A significantly greater change in resorption area was detected in cultures in which osteoclast formation was supported by oim/oim mCOB. Data are obtained from three independent biological experiments. *P ≤ 0.05, **P ≤ 0.01.