Figure 9.

Effect of MAT1A overexpression on ERK and AKT activation in vitro and in vivo. Western blotting for phospho-ERK (p-ERK), phospho-AKT (p-AKT), and total ERK and AKT was performed in (A) MAT1A transfected Huh7 cells or (B) MAT1A overexpressing tumors as described in Materials and Methods. Densitometric ratios of phospho to total protein in MAT1A transfectants are expressed as fold over empty vector. Results represent mean ± SEM from three independent experiments. *P < 0.05; **P < 0.005 versus empty vector cells.