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. 2010 May;176(5):2500–2508. doi: 10.2353/ajpath.2010.091041

Figure 3.

Figure 3

Reduction of IKKα expression suppresses UVB-induced apoptosis in mouse skin and promotes cell proliferation. A and B: Paraffin-embedded skin sections obtained from Ikkα+/+ (+/+) and Ikkα+/− (+/−) mice using a TUNEL assay kit and the number of TUNEL-positive cells determined under a microscope. Groups of mice (n = 3) used. The SBCs in the epidermis of hematoxylin and eosin–stained skin sections were counted. 24 hours post-UVB, skin sections prepared from mice at 24 hours after UVB irradiation at 100 mJ/cm2. **P < 0.01, t test. C: Levels of p-ERK and ERK in skin specimens obtained from UVB-irradiated mice detected using Western blotting. β-Actin, protein loading controls; C, untreated sample; 6 hours and 24 hours, samples prepared from mice at 6 hours and 24 hours post-UVB irradiation (100 mJ/cm2). D: Comparison of Ki-67–positive cells immunohistochemically stained in the interfollicle epidermis of mice. Groups of mice (n = 4) used. 2-w post-UVB, skin sections prepared from mice at week 2 after treatment with UVB at 100 mJ/cm2. **P < 0.01, t test.