Table 1. List of PCR primers for LOXL1 (lysyl oxidase-like 1) exon sequencing in South African black individuals with or without exfoliation glaucoma.
| LOXL1 exon | Forward primer sequence | Reverse primer sequence | PCR product size (bp) | Covered genomic region* |
|---|---|---|---|---|
| Promoter |
CCACCAACAAAGAGGGTGTG |
ACCGCCTGTGGGCCTTAC |
597 |
chr15:72,005,330–72,005,926 |
| Exon1a |
TCCCAGCCTGTTGCTTATTC |
AGGCCTGGTGGACAGAGAG |
326 |
chr15:72,005,836–72,006,161 |
| Exon1b |
AAGCAAGGAGCCTTCCTGTC |
GCACCCGGGAGCTACTCT |
330 |
chr15:72,006,074–72,006,403 |
| Exon1c1 |
GCAGGTGTACAGCTTGCTCA |
ACACGAAACCCTGGTCGTAG |
464 |
chr15:72,006,324–72,006,787 |
| Exon1c2 |
GCTCAACTCGGGCTCAGA |
GAACTGCTGCGGGTAGGA |
370 |
chr15:72,006,339–72,006,708 |
| Exon1d |
CTCCTACCCGCAGCAGTTC |
GGTACTCGGGCAGCTCTTC |
227 |
chr15:72,006,690–72,006,916 |
| Exon1e |
CGACCAGGGTTTCGTGTACT |
AGGTAGGGCGGCTCCAG |
402 |
chr15:72,006,771–72,007,172 |
| Exon1f |
AGCAGGCCTACCCTGACC |
GCCTCCAGGAAGTTCTAAGGA |
340 |
chr15:72,007,037–72,007,376 |
| Exon2 |
CCAACCTGATGCTCTCAATG |
CACAGCTAGGCTGGGTTCTG |
248 |
chr15:72,022,182–72,022,429 |
| Exon3 |
CATGCTGGGTTCTGGTGTC |
GAGCTCAGGCACCAAGGTC |
271 |
chr15:72,025,749–72,026,019 |
| Exon4 |
CAGGGAAGACTAGGCCCTCT |
CTGTGAGCAGAGCTGAGTGG |
324 |
chr15:72,026,413–72,026,736 |
| Exon5 |
CCAGAAACTCCTGAAGGTGG |
GGGACATTGGACATGAACATC |
232 |
chr15:72,027,121–72,027,352 |
| Exon6 |
TTACCACCTTCTCTGGTGAGC |
TCCCCAGGCAGGAAAGG |
248 |
chr15:72,028,773–72,029,020 |
| Exon7 | CCCTCATTGACCCACTGTCT | GCATGCAGAGCCACAGAGTA | 356 | chr15:72,031,193–72,031,548 |
The asterisk indicates the covered genomic regions were based on the March 2006 human reference sequence (NCBI Build 36.1).