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(A) IFA and confocal microscopy show that gμ2 localizes predominantly to the peripheral vacuoles (PVs). (B) Live trophozoites were incubated with the lysosomal marker LysoTracker (red), fixed, labelled with anti-gμ2 mAb (green), and analyzed by confocal microscopy. The merged image shows colocalization (yellow) in the PVs. Some cells show colocalization in the bare zone. Bar, 10 μm.
