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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem J. 2010 Apr 28;428(1):33–45. doi: 10.1042/BJ20100096

Figure 4. gAP2 localizes to both the lysosome-like peripheral vacuoles and the plasma membrane.

Figure 4

(A) (a) Electromicrograph of a growing Giardia trophozoite showing the PVs located underneath the plasma membrane (PVs, arrows). Nuclei are also denoted. Bar, 0.5 μm. (b–f) Enlarged immunoelectromicrograph of the PVs. gμ2 (red arrowheads) is detected on the inner side of the plasma membrane and on the PVs. (g) Enlarged electromicrograph of unstained PVs. (h) Electromicrograph of the control using secondary antibody alone. PM: plasma membrane. G: electron-dense glycogen deposits. Bar, 0.1 μm. (B) IFA and confocal microscopy show gμ2 (red) and clathrin (green) in the PV area (Merge-Colocalization). Bar, 10 μm.