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Uptake assays of fluorophore-labelled LDL (BODIPY-LDL) and dextran (FITC-dextran) demonstrate that both the LDL and dextran localize to the PVs in non-permeabilized trophozoite (left panels, −ds). When gμ2 is depleted, the LDL remains on the surface, including the flagella (arrowheads), while dextran is still internalized to the PVs (right panels, +ds). Bar, 10 μm.
