Table 1.
Primer sequences and PCR product sizes of wild type and internal control DNAs.
| ABL: | ||
| F-ABL-A2: | 5′-TGA CTT TGA GCC TCA GGG TCT GAG TGA AGC C-3′ | |
| R-ABL-A3: | 5′-CCA TTT TTG GTT TGG GCT TCA CAC CAT TCC-3′ | |
| Annealing temperature: | 70°C | |
| Reference sequence (mRNA): | GenBank:NM_007313 | |
| Reference sequence (genomic DNA): | GenBank:U07563 | |
| Nucleotide position (mRNA): | 159–374 | |
| Nucleotide position (genomic DNA): | 49914–50692 | |
| RT-PCR product: | 216 bp | |
| PCR product: | 779 bp | |
| Taq polymerase: | TaKaRa ExTaq HS (Lonza) | |
| EBV: | ||
| F-EBV (TC-70): | 5′-CTT GGA GAC AGG CTT AAC CAG ACT CA-3′ | |
| R-EBV (TC-72): | 5′-CCA TGG CTG CAC CGA TGA AAG TTA T-3′ | |
| Annealing temperature: | 60°C | |
| Reference sequence: | GenBank: V01555 | Nucleotide positions: 83520–83784 |
| PCR product (wild type): | 265 bp | PCR product (internal control): 445 bp |
| Taq polymerase: | TaKaRa Taq HS (Lonza) | |
| HBV: | ||
| F-HEPB: | 5′-AAG CTG TGC CTT GGG TGG CTT-3′ | |
| R-HEPB: | 5′-CGA GAT TGA GAT CTT CTG CGA C-3′ | |
| Annealing temperature: | 55°C | |
| Reference sequence: | GenBank: NC_003977 | Nucleotide positions: 1874–2436 |
| PCR product (wild type): | 563 bp | PCR product (internal control): 906 bp |
| Taq polymerase: | BioTherm Taq polymerase (Gene Craft, Lüdinghausen, Germany) | |
| HCV: | ||
| F-HCV: | 5′-GCC ATG GCG TTA GTA TGA GT-3′ | |
| R-HCV: | 5′-RTG CAC GGT CTA CGA GAC CT-3′ | |
| Annealing temperature: | 55°C | |
| Reference sequence: | GenBank: NC_004102 | Nucleotide positions: 82–340 |
| PCR product (wild type): | 259 bp | PCR product (internal control): 452 bp |
| Taq polymerase: | TaKaRa ExTaq HS (Lonza) | |
| HHV-8: | ||
| F-HHV-8: | 5′-AGC CGA AAG GAT TCC ACC AT-3′ | |
| R-HHV-8: | 5′-TCC GTG TTG TCT ACG TCC AG-3′ | |
| Annealing temperature: | 56°C | |
| Reference sequence: | GenBank: NC_009333 | Nucleotide positions: 47386–47618 |
| PCR product: | 232 bp | |
| Taq polymerase: | TaKaRa ExTaq HS (Lonza) | |
| HIV-1: | ||
| F-HIV (SK38): | 5′-ATA ATC CAC CTA TCC CAG TAG GAG AAA T-3′ | |
| R-HIV (SK39): | 5′-TTT GGT CCT TGT CTT ATG TCC AGA ATG C-3′ | |
| Annealing temperature: | 50°C | |
| Reference sequence: | GenBank: NC_001802 | Nucleotide positions: 1090–1204 |
| PCR product (wild type): | 114 bp | PCR product (internal control): 403 bp |
| Taq polymerase: | TaKaRa Taq HS (Lonza) | |
| HTLV-I/-II: | ||
| F-HTLV (SK110): | 5′-CCC TAC AAT CCA ACC AGC TCM G-3′ | |
| 5′-CCT TAC AAT CCA ACC AGC TCA G-3′ | ||
| 5′-CCA TAC AAC CCC ACC AGC TCA G-3′ | ||
| R-HTLV (SK111): | 5′-GTG RTG GAT TTG CCA TCG GGT T-3′ | |
| 5′-GTG GTG AAG CTG CCA TCG GGT T-3′ | ||
| Annealing temperature: | 60°C | |
| Reference sequence: | GenBank: NC_001436 | Nucleotide positions: 4384–4569 |
| PCR product (wild type): | 186 bp | PCR product (internal control): 405 bp |
| Taq polymerase: | TaKaRa Taq HS (Lonza) | |
| SMRV: | ||
| F-SMRV-env: | 5′-GGC GGA CCC CAA GAT GCT GTG-3′ | |
| R-SMRV-env: | 5′-TGG GCT AGG CTG GGG TTG GAG ATA-3′ | |
| Annealing temperature: | 67°C | |
| Reference sequence: | GenBank: M23385 | Nucleotide positions: 6857–7053 |
| PCR product: | 197 bp | |
| Taq polymerase: | TaKaRa Ex Taq HS (Lonza) | |
| F-SMRV-gag: | 5′-TCA GAG CCC ACC GAG CCT ACC TAC-3′ | |
| R-SMRV-gag: | 5′-CAG CGC AGC ACG AGA CAA GAA AA-3′ | |
| Annealing temperature: | 67°C | |
| Reference sequence: | GenBank: M23385 | Nucleotide positions: 1737–1922 |
| PCR product: | 186 bp | |
| Taq polymerase: | TaKaRa Taq HS (Lonza) | |