Table 2.
EBV-PCR+ cell lines, BZLF1 expression detected by Western blotting, differentiation of episomal and linear EBV-DNA, EBV sequence pattern detected by Southern blotting, and episomal load determined by FISH.
| Cell line | Cell type | PCRa | BZLF1 expr.a | In situ lysing gelb | Southern blotc | FISHd | ||
|---|---|---|---|---|---|---|---|---|
| untr. | TPA | episomal | linear | |||||
| ARH-77 | B-LCL | + | − | − | (+) | − | + | + |
| B95-8 | marmoset monkey lymphocytes | + | + | + | ++ | +++ | + | +++ |
| BD-215 | B-LCL | + | + | + | + | + | + | ++ |
| BONNA-12 | hairy cell leukemia | + | − | − | ++ | + | + | ++ (h) |
| CI-1 | B cell lymphoma | + | − | − | + | − | n.d. | + |
| COLO-720L | B-LCL | + | − | − | + | + | + | ++ |
| CRO-AP2 | B cell lymphoma | + | − | − | (+) | − | + | ++ |
| CRO-AP5 | B cell lymphoma | + | − | − | (+) | − | + | ++ |
| DAUDI | Burkitt lymphoma | + | − | − | +++ | + | + | ++ (h) |
| DOHH-2 | B cell lymphoma | + | − | − | − | − | − | + (f) |
| EB-1 | Burkitt lymphoma | + | − | + | + | + | + | ++ (h) |
| EHEB | chronic B cell leukemia | + | − | − | + | (+) | + | n.d. |
| GRANTA-519 | B cell lymphoma | + | − | + | ++ | ++ | + | ++ |
| HC-1 | hairy cell leukemia | + | − | + | ++ | (+) | + | ++++ |
| IM-9 | B-LCL | + | − | + | +++ | − | + | ++++ |
| JIYOYE | Burkitt lymphoma | + | − | + | ++ | ++ | + | +++ |
| JVM-13 | chronic B cell leukemia | + | − | − | ++ (2 bands) | − | + | ++++ |
| JVM-2 | chronic B cell leukemia | + | − | − | + | + | + | +++ |
| JVM-3 | chronic B cell leukemia | + | − | − | ++ | + | + | ++ |
| L-591 | B-LCL | + | + | + | ++ | ++ | + | +++ |
| LCL-HO | B-LCL | + | − | − | ++ | (+) | + | +++ |
| LCL-WEI | B-LCL | + | − | − | + | + | + | ++ |
| MEC-1 | chronic B cell leukemia | + | − | − | + | + | + | ++ (h) |
| MEC-2 | chronic B cell leukemia | + | − | − | + | − | + | ++ (h) |
| NAMALWA | Burkitt lymphoma | + | − | − | − | − | (+) | − |
| NAMALWA.CSN/70 | Burkitt lymphoma | + | − | − | − | − | (+) | − |
| NAMALWA.IPN/45 | Burkitt lymphoma | + | − | − | − | − | (+) | − |
| NAMALWA.KN2 | Burkitt lymphoma | + | − | − | − | − | (+) | − |
| NAMALWA.PNT | Burkitt lymphoma | + | − | − | − | − | (+) | − |
| NC-NC | B-LCL | + | + | + | ++ | ++ | + | +++ |
| NK-92 | natural killer lymphoma | + | − | − | + | + | + | + |
| OCI-LY-19 | B cell lymphoma | + | − | − | − | − | − | − |
| RAJI | Burkitt lymphoma | + | − | − | +++ | + | + | ++++ |
| RO | B-LCL | + | − | − | + | − | + | ++ |
| SD-1 | B-LCL | + | − | − | ++ | + | + | +++ |
| T2 | human-human somatic cell hybrid | + | − | − | ++ | + | n.d. | n.d. |
| TMM | B-LCL | + | − | − | + (2 bands) | (+) | + | +++ |
| VAL | B cell lymphoma | + | − | + | ++ (2 bands) | + | n.d. | ++ (h) |
| YT | T/natural killer cell leukemia | + | + | + | ++ | +++ | + | +++ |
B-LCL: B-lymphoblastoid cell line; expr.: expression; n.d.: not determined; untr.: untreated.
a−: negative; +: positive.
b−: negative; (+): weakly positive; +: positive; ++: strongly positive; +++: very strongly positive.
c −: no bands visible; (+): very weak signals; +: clear bands visible.
d episomal load detected by FISH: −: no episomes; +: low load; ++: load; +++: high load; ++++: very high load; (h): heterogenous distribution of the episomes among the cells; (f): only a few cells containing episomes.