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. 2010 May 1;24(9):875–880. doi: 10.1101/gad.565010

Figure 3.

Figure 3.

Abnormal lymphatic vessels in the Clp24 gene targeted mice. (A) Whole-mount staining of lymphatic and blood vessels for LYVE-1 and PECAM-1, respectively, in the ears of Clp24−/− and Clp24+/− mice. (B,C) Paraffin sections of the tongue (B) and the small intestine (C) of wild-type, Clp+/−, and Clp24−/− mice were stained for LYVE-1. Note enlarged lymphatic vessels in Clp24−/− mice (arrowheads in A–C). (D,E) Whole-mount staining of the ears of Clp24−/− (D) and wild-type (Clp24+/+) (E) mice for VEGFR-2 and SMA to detect perivascular SMCs. Note enlarged SMC-covered collecting lymphatic vessels (arrows) in Clp24−/− mice. (F) Quantification of SMC-covered initial lymphatic vessels in the ear periphery in Clp24−/− and wild-type mice. Shown is the mean of lymphatic vessel-associated SMC clusters per 2.25-mm2 field. Representative images used for quantification are shown in Supplemental Figure S6, C and D. Bar, 100 μm.