piRNA expression analysis in the MVH-deficient fetal testis by deep sequencing. (A) The size distribution of the total small RNAs in E16.5 testes. Total numbers of small RNAs sequenced from wild-type and MVH-deficient testis libraries were 2,090,035 and 2,812,945, respectively. (B) Ratio of total and repeat-associated piRNA to miRNA. (C,D) Ratio of the first (C) and 10th (D) nucleotides of the repeat-associated piRNAs. (E) Expression of piRNA cluster-derived piRNAs in the MVH-deficient testes. The data of piRNAs derived from the large 19 piRNA clusters are shown. The ratios of piRNAs in the MVH-deficient testes per those in wild-type testes are shown. (Supplemental Table 2) Number 1 represents the data of the largest piRNA cluster, and the following are ordered according to the sizes of the clusters.