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Nkx2.5 expression in calcium-induced keratinocyte differentiation. The protein from cultured keratinocyte extracts of 20 or 30 µg was loaded for SDS-PAGE, and was detected with peptide polyclonal anti-Nkx2.5 antibody, anti-involucrin. Secondary antibodies were obtained from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Involucrin used as a control marker for differentiation was also increased with calcium treatment. Actin was used as a loading control.
