Nkx2.5 expression in several skin diseases. Immunohistochemical staining was performed using Nkx2.5 antibody, at least 3 samples of each disease was studied. The Nkx2.5 expression generally observed in nuclei and/or cytoplasm in various skin tissues. Normal negative means that normal skin was stained in the same procedure only without Nkx2.5 antibody. (A) Normal negative (H&E, ×100), (B) Normal skin (H&E, ×100), (C) Psoriasis (H&E, ×200), (D) AD (H&E, ×200), (E) AK (H&E, ×200), (F) SCC (H&E, ×100), (G) SCC (H&E, ×200), (H) BCC (H&E, ×100), (I) BCC (H&E, ×200). AD: atopic dermatitis, AK: actinic keratosis, SCC: squamous cell carcinoma, BCC: basal cell carcinoma.