Table 5. Changes in gene expression by NSAIDs in DU-145 cells.

Real-Time RT- PCR analysis of GDF 15, angiopoietin-like4 (ANGPTL4) and E2F2 mRNA in DU-145 cells treated with NS-398 (0.01mM and 0.1mM) and ibuprofen (0.1mM and 1.5mM). Each data point represents Average ± SEM of 3 separate experiments.

Treatment	COX-2	NAG-1	ANGPTL4	E2 F2	MMP 3
DMSO Control	1	1	1	1	1
0.01mM NS-398	1.1 ± 0.0**	1.0 ± 0.1	1.2 ± 0.1	1.3 ± 0.1*	1.1 ± 0.1
0.1mM NS-398	2.5 ± 0.2*	6.7± 0.4**	2.0 ± 0.3	0.9 ± 0.1	1.9 ± 0.2*
Control	1	1	1	1	1
0.1mM ibuprofen	1.3 ± 0.1*	0.7 ± 0.2	6.2 ± 0.9*	1.0 ± 0.3	1.9 ± 0.2
1.5mM ibuprofen	25.0 ± 0.9**	106.1 ± 10.1*	39.5 ± 3.7*	0.4 ± 0.1*	149.4 ± 0.1*

^*p<0.05 and

^**p<0.005