FIGURE 2.

Splenic CD8 T_CM down-regulate CD27 and acquire granzyme B after entry into nonlymphoid tissues. OT-I transferred CD45.2 mice were infected with 1 × 10³ CFU of LM-ova i.v. 48 days later mice were re-infected with 1 × 10⁶ PFU VSV-ova i.v., and 97 days later, splenocytes were isolated, enriched for CD8 T cells, donor cells were sorted into either CD62L^low/CD27^high cells (A) or CD62L^high/CD27^high cells (B) and transferred to naive CD45.2 mice (1×10⁶ and 5×10⁵, respectively). Four days later lymphocytes were isolated from the spleen, lung, and liver, and the level of CD27, CD62L, and granzyme B expression by the transferred OT-I T cells was determined. C, OT-I transferred mice were infected with LM-ova i.v.; 46 days later mice were infected with VSV-ova i.v., and 58 days later splenocytes were isolated, enriched for CD8 T cells, donor cells were sorted, and 8.5 × 10⁵ CD27^high cells were transferred to naive CD45.2 mice. On days 0, 1, and 2, mice were treated with either 250 µg of anti-CD70 (FR70) or control rat IgG. Four days later, lymphocytes were isolated from the spleen, lung and liver, and the level of CD27 was determined on the transferred OT-I T cells. Values indicate the MFI of staining for the population. For GrB staining the MFI is shown for the isotype control and test, respectively. Data are from one representative animal of 1–4 mice analyzed from four experiments (n = 9).