Taf3 is required for normal development and hematopoiesis in early zebrafish embryos. A: RT-PCR analysis monitoring expression of taf3 and, as a control, gapdh in zebrafish embryos at 0, 4, 8 and 24 hpf. B: Immunoblot analysis monitoring expression of Taf3 in zebrafish embryos at 0, 4, 6, 18 and 24 hpf. C: Whole-mount in situ hybridization with a riboprobe to taf3 at 6 and 24 hpf. D: Phenotypic analysis of embryos injected with a control, taf3, trf3 or cdx4 MO at 24 hpf. E: (Top) Whole-mount in situ hybridization with riboprobes to gata1 and pax2 (14 hpf). (Bottom) Fluorescence microscopy monitoring expression of an gata1:dsRed transgene at 30 hpf. F: Whole-mount in situ hybridization with riboprobes to scl and hbbe1 (18 hpf).