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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Nat Med. 2009 Dec 20;16(1):123–128. doi: 10.1038/nm.2071

Figure 2.

Figure 2

CD27+ memory peripheral blood cells acquire a stable GC-like phenotype following transduction with BCL6 and Bcl-xL and subsequent culture. (a) Phenotype of BCL6+Bcl-xL transduced CD27+ memory B cells (6XL, black histogram line) compared to tonsil GC cells (GC, CD38+CD20+, shaded grey), tonsil naïve and memory cells (N/M, CD38−CD20low, red histogram), and tonsil plasma cells (PC, CD38++CD20low, blue histogram). BCL6+Bcl-xL transduced monoclonal cell lines show an identical phenotype (not shown). (b) Relative mRNA levels of AICDA (encoding AID) in CD19+IgG+CD27+ PB memory B cells and CD19+CD38+CD20+IgD− tonsillar GC B cells (value set as 1) compared to BCL6+Bcl-xL transduced bulk CD27+ memory cells using quantitative 5 RT-PCR.