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. 2010 May;82(5):855–860. doi: 10.4269/ajtmh.2010.09-0533

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Figure 2. — Loop-mediated isothermal amplification (LAMP) reactions with the snoRNA primer set for amplification of Trypanosoma rangeli DNA. Standard positive reaction threshold is 0.1 of the value of the turbidity released. (A) Specificity test of LAMP assay using the real-time turbidimetry device. Tran – T. rangeli; Tcru – Trypanosoma cruzi; Tinf – Triatoma infestans; Rpro – Rhodnius prolixus; Human – DNA extracted from uninfected human blood; and DW – distilled water used as non-DNA negative control. Sensitivity test on serially diluted T. rangeli DNA from 100 ng down to 1 fg: (B) detection using the real-time turbidimetry device, (C) detection under UV light by the naked eye using FD reagent. The green/bright fluorescence indicates a positive reaction and the dark/less fluorescent color indicates a negative reaction. This figure appears in color at www.ajtmh.org.