Table 2.
Insect stage | Total no. of samples | T. rangeli | T. cruzi | ||
---|---|---|---|---|---|
PCR | LAMP | PCR | LAMP | ||
+ve* | +ve | +ve | +ve | ||
Nymph | 37 | 0 (0%) | 3 (8%) | 7† (19%) | 10† (27%) |
Adult | 15 | 4‡ (27%) | 4‡ (27%) | 7‡ (47%) | 7‡ (47%) |
Total | 52 | 4 (8%) | 7 (13%) | 14 (27%) | 17 (33%) |
Positive detection.
Both loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) positively detected T. cruzi from same seven samples, whereas 3 samples were positive by LAMP only.
Four samples were detected as T. cruzi and T. rangeli mixed infections by both LAMP and PCR.