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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: Cell. 2009 Jul 10;138(1):78–89. doi: 10.1016/j.cell.2009.06.029

FIGURE 6. SLX4 associates with MUS81-EME1.

FIGURE 6

A- Table summarizing the number of peptides and the percentage of coverage obtained by Mass Spectrometric analysis of a FLAG-EME1 precipitate.

B- Western blot detection of endogenous MUS81 in total cell lysates of HEK cells co-producing FLAG-SLX1 and GFP-tagged full length or C-terminal SLX4 fragments and the corresponding FLAG-eluates. These are the same total cell lysates and FLAG-eluates than the ones described in Figure S10. The FLAG-eluates were also used in the nuclease assay shown in Figure 4E. The amount of total cell lysate loaded per lane is 10% of the input relative to the amount loaded of the corresponding FLAG-eluate.

C- and D- HEK cells were transfected with pcDNA3 constructs expressing the indicated deletion constructs of MUS81 or EME1 and full length SLX4. Cell lysate and α-FLAG immunoprecipitates (IP) were analyzed by western blot with the indicated antibodies.