. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Pharm Res. 2009 Aug 14;26(10):2358–2366. doi: 10.1007/s11095-009-9952-9

Table I.

Cysteine Scanning Mutants of TMD10 of hPEPT1

Mutation	Codon change
Wild Type	NA
I585C	CCG to TGC
P586C	CAG to TGC
Q587C	GAG to TGC
Y588C	TAT to TGT
F589C	TTT to TGT
L590C	CTT to TGT
L591C	CTC to TGC
T592C	ACC to TGC
G594C	GGC to TGC
E595C	GAA to TGC
V596C	GTC to TGC
V597C	GTC to TGC
F598C	TTG to TGC
S599C	TCT to TGT
V600C	GTC to TGC
T601C	ACG to TGC
G602C	GGA to TGC
L603C	TTG to TGC
E604C	GAA to TGC
F605C	TTC to TGC

A series of 20 cysteine-scanning mutant cDNAs was created by oligonucleotide-mediated site-directed mutagenesis of WT-hPEPT1 cDNA. Each of the 20 amino acid residues within TMD10 was individually mutated to a cysteine.