Figure 2.

Mn Concentration Is Altered in the nramp1-1 Mutant.

Elemental analysis was performed by ICP-MS on roots and shoots of wild-type or nramp1-1 plants grown for 2 weeks in vitro either in Mn-replete (20 μM MnSO₄) or in Mn-free (no added Mn) medium as indicated in the figure. Data are from one representative experiment (n = 2). Error bars represent sd. *, Significant difference between the wild type and nramp1-1, P < 0.01, n = 5 (Student's t test). DW, dry weight.

(A) and (B) Mn content.

(C) and (D) Fe content.