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. 2010 Mar 12;22(3):904–917. doi: 10.1105/tpc.109.073023

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© 2010 American Society of Plant Biologists

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Figure 7. — NRAMP1 Partially Rescues irt1-1 Harboring a Broad Metal Selectivity in Vivo.

(A) irt1-1/ProIRT1:HA-NRAMP1 transgenic lines #1 and 3 produce the HA-NRAMP1 fusion protein in Fe-deficient roots. Plants were grown in vitro in Fe-replete (50 μM FeEDTA) or Fe-limited (10 μM Fe-EDTA) medium for 2 weeks. Total protein extracts were immunoblotted and hybridized to an anti-HA antibody.

(B) and (C) NRAMP1 partially restores growth of irt1-1 under Fe deficiency.

(B) Phenotype of the wild type, irt1-1, and irt1-1/ProIRT1:HA-NRAMP1 line #1 grown in soil for 5 weeks.

(C) Root and shoot biomass of the wild type, irt1-1, or irt1-1/ProIRT1:HA-NRAMP1 lines #1 and 3 grown in vitro in Fe-limited conditions as in (A).

(D) NRAMP1 partially rescues Fe, Co, and Zn uptake defects of the irt1-1 mutant. Elemental analysis performed by ICP-MS on roots of the wild type, irt1-1, or irt1-1ProIRT1:HA-NRAMP1 lines #1 and 3 grown in vitro for 10 d in metal replete medium and then transferred to Fe-free (no added Fe) or Mn-limiting (0.5 μM MnSO₄) conditions for five additional days. Error bars represent sd. Significant differences between irt1-1 and the complemented lines are indicated by * P < 0.05 and ** P < 0.01. n = 4 (C) or n = 10 (D). One representative experiment (n = 2) is shown in (C) and (D). DW, dry weight.