Table 1.
Mn |
Fe |
Co |
Zn |
Cu |
||||||||
Condition | Genotype | Tissue | Mean | sd | Mean | sd | Mean | sd | Mean | sd | Mean | sd |
Standard | wild type | shoot | 418 | 22 | 100 | 2 | 0.66 | 0.04 | 368 | 15 | 6.6 | 0.2 |
nramp1-1 | 447 | 25 | 103 | 2 | 0.68 | 0.08 | 367 | 29 | 6.8 | 0.2 | ||
wild type | root | 290 | 39 | 446 | 86 | 2.87 | 0.32 | 1904 | 282 | 13.3 | 2.1 | |
nramp1-1 | 126 | 49 | 680 | 170 | 1.73 | 0.49 | 1293 | 236 | 18.3 | 6.7 | ||
0 Mn | wild type | shoot | 13 | 1 | 116 | 7 | 0.26 | 0.01 | 247 | 15 | 6.8 | 0.6 |
nramp1-1 | 4 | 0 | 130 | 19 | 0.16 | 0.01 | 204 | 32 | 9.3 | 0.6 | ||
wild type | root | 6 | 2 | 1620 | 202 | 0.45 | 0.07 | 640 | 185 | 11.8 | 2.3 | |
nramp1-1 | 5 | 2 | 1836 | 49 | 0.42 | 0.13 | 1361 | 686 | 28.0 | 11.3 |
ICP-MS measurement of roots and shoots of wild-type or nramp1-1 plants grown for 2 weeks in vitro either in standard Mn (20 μM MnSO4) or in Mn-free (no added Mn) medium as indicated (n = 5). Significant changes between the wild type and mutant are indicated in bold.