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. 2010 Mar 30;22(3):703–715. doi: 10.1105/tpc.109.071407

Figure 4.

Figure 4.

The Expression of PIF4 and HFR1 Was Altered in Transgenic Plants That Overexpress Either the SPX or the EXS Domain.

(A) and (B) The expression of PIF4 (A) and HFR1 (B) in Ws, shb1-D, or transgenic plants that overexpress either N320:GFP (line 2) or C225:GFP (line 1). Total RNA was isolated from 5-d-old seedlings grown in the dark or in the dark then treated for 4 h with red, far-red, or blue light. The expression levels of PIF4 or HFR1 were normalized to that of UBQ10. Data are presented as means plus or minus the se from two independent experiments.

(C) Hypocotyl elongation responses of Ws, pif4, or transgenic plants that express N320:GFP in either the Ws or pif4 background in the dark or under red or blue light.

(D) Hypocotyl elongation responses of Col, hfr1, or transgenic plants that express C225:GFP in either the Col or hfr1 background in the dark or under blue light. The light intensities were as specified in Figure 1C. Data are presented as means plus or minus the se from at least 25 seedlings.