The SPX and EXS Domains Participate in the Formation of an Altered Protein Complex.
(A) The expression of SHB1:GFP, N320:GFP, and C225:GFP in two independent transgenic lines as detected by immunoblot analysis using anti-GFP antibody.
(B) Gel filtration profiles of endogenous SHB1 and SHB1:GFP in transgenic plants that overexpress SHB1:GFP in the Ws background.
(C) Gel filtration profiles of SHB1:GFP (line 5) and N320:GFP (line 2) in Ws or shb1, or C225:GFP (line 1) in Ws or shb1.
(D) Gel filtration profiles of endogenous SHB1 in Ws and transgenic plants that overexpress either N320:GFP (line 2) or C225:GFP (line 1) in Ws background. Plant extracts from 7-d-old seedlings were fractionated on a Superdex-200 gel-filtration column. The various fractions were collected, separated by SDS-PAGE, and blotted with anti-GFP antibodies or antisera against SHB1. The molecular mass standards of the gel filtration fractions are labeled at the bottom, and the molecular mass markers of the SDS-PAGE gel are labeled on the left.