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. 2010 Mar 30;22(3):703–715. doi: 10.1105/tpc.109.071407

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Figure 7. — Overexpression of SHB1 with a G81R Conversion Fails to Create a Long Hypocotyl Phenotype.

(A) Expression of SHB1:GFP in transgenic plants overexpressing wild-type SHB1:GFP (line 5) or mutated SHB1:GFP G81R (lines 2 and 6) as immunoblotted with anti-GFP antibody.

(B) Hypocotyl elongation responses of Ws, SHB1 OE transgenic line 5, and two independent transgenic plants that overexpress mutated SHB1 G81R (lines 2 and 6) in the dark or under red light (top panel), and far-red or blue light (bottom panel) at intensities as specified in Figure 1C. The means plus or minus se were calculated from at least 25 seedlings. OE indicates overexpression.