Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Mar 9;22(3):755–771. doi: 10.1105/tpc.109.071746

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 American Society of Plant Biologists

PMC Copyright notice

Figure 9. — Comparative Analysis of MAP65 Isoform Composition and Expression Levels.

(A) and (B) General immunoblot survey of MAP65 isoform composition detectable with an anti-CEEESWLEDYNR antibody (A) or with isoform-specific anti-MAP65-1 and anti-MAP65-3 antibodies (B) showing quantitative upregulation of the MAP65-1 and downregulation of the MAP65-3 in both the anp2 anp3 and the mpk4 mutants compared with the respective Ws and Col-0 controls. Numbers indicate molecular mass in kilodaltons. The star denotes a band probably corresponding to a degradation product of MAP65. Anti-actin staining was used as a loading control (B).

(C) RT-PCR analysis of MAP65-1 and MAP65-3 transcript levels (26 amplification cycles) showing no visible differences between Ws, anp2 anp3, Col-0, and mpk4 plants. ACTIN2 transcript (26 amplification cycles) was used as a loading control. Three biological and three technical replicates were performed.