Table 2.
Density of Labeling by Antibodies against VIG1 among Organelles
| Number of Gold Particles (100 nm−2) |
||
| Organelle | Membranea | Inner Regionb |
| Vacuoles | 22.0 ± 3.0 | 0.0 ± 0.0 |
| Nuclei | 1.9 ± 0.5 | 1.1 ± 0.3 |
| Mitochondria | 2.1 ± 0.7 | 0.3 ± 0.1 |
| Plastids | 0.2 ± 0.0 | 0.7 ± 0.1 |
| Cytosol | 0.0 ± 0.0 | 0.3 ± 0.2 |
Numbers of immunogold particles labeling VIG1 on each organelle of sections were counted.
a
Membrane region was defined as the line of membrane in the image and 50 nm outside of it.
b
Inner region includes the inside of the organelle membrane or cytoplasmic membrane. Immunogold particles of VIG1 selectively localized on the vacuolar membrane. Values are means ± se. n = 50, number of cell sections examined.