Table 3.

Density of Labeling by Antibodies against VIG1 on Membranes of Vacuoles Tethered to Mitochondria

Organelles	Number of Gold Particles (100 nm−2)	P Value in Welch's t Test
Vacuolar membrane
Cytosol sidea	2.0 ± 0.7	6.2 × 10−5 d
Mitochondrial sideb	27.8 ± 1.9	–
Mitochondrial membranec	1.7 ± 0.4	5.8 × 10−5 e

Numbers of immunogold particles on the parts of the vacuoles bound to the mitochondria were counted. The area of the vacuole was divided into a and b. a, The two-thirds of the vacuolar membrane toward the cytosol; b, the one-third of the vacuolar membrane facing the mitochondria. Mitochondrial membrane (c) was used as a control. Divided areas of the vacuole membrane are shown in a schematic diagram (see Supplemental Figure 4 online). Welch's t test showed a significant difference of density of immunogold particles of VIG1 among the mitochondrial side on the vacuole (b) and the other area (a or c). d, Welch's t test for b versus a. e, Welch's t test for b versus c. VIG1 accumulated on the mitochondrial side of the vacuole membrane. Values are means ± se. n = 50, number of cell sections examined.