Figure 7.

Activity of Paralogous GER4 Promoters in Response to Pathogen Attack.

(A) The different full-length GER4 promoter sequences (∼3 kb) were fused to the wheat WIR1a intron and the GUS reporter for transient expression experiments. The reporter constructs were introduced into barley epidermal cells by particle bombardment. GUS-positive cells were counted in the presence (+) or absence (−) of Bgh spores 48 h after inoculation. The GUS expression in each experiment was normalized to a cobombarded CaMV 35S:GFP construct (pGFP). Mean values and se of six independent experiments is shown. ter, wheat GstA1 terminator.

(B) Schematic representation of the W-boxes (diamonds; [C/T]TGAC[T/C]) in the full-length promoter region of the paralogous GER4 genes. Distances are given relative to the transcriptional start site.