Table 1.
Boechera Accessions Used in SuperSAGE Analysis, Including Stage-Specific Developmental Characteristics and Ploidy Content of Cells in Ovules Microdissected from Sexual and Apomictic Boechera
| Sample | Speciesa | ID | Reproductionb | Stagec | Developmentd | Microdissected Ovule Ploidy Compositiond | Collection Locality |
| Sex A | B. polyantha (B. holboellii) | 105.6 | Sex | A | Nucellus | Diploid | Bandy Ranch, Missoula Co, Montana |
| Sex B | B | MMC formation | Haploid, diploid | ||||
| Sex C | C | Tetrad to degeneration | Haploid, diploid | ||||
| Sex D | D | Fertilized ovules | Diploid, triploid | ||||
| Apo A | B. retrofracta (B. divaricarpa) | 67.5 | Apomixis | A | Nucellus | Diploid | Ranch Creek, Granite Co, Montana |
| Apo B | B | MMC formation | Diploid | ||||
| Apo C | C | Tetrad to degeneration | Diploid | ||||
| Apo D | D | Fertilized ovules | Diploid, tetraploid, hexaploid |
Species identifications were based upon silique orientation, trichome morphology, and cpDNA sequences (see Kiefer et al., 2009). In parentheses are old species names for the same plants which were used in Sharbel et al. (2009).
Reproductive mode was confirmed using the flow cytometric seed screen (Matzk et al., 2000; Sharbel et al., 2009).
See Figure 1 for images of each stage.
According to Schneitz et al. (1995)